TMDb/Spider-Noir Celebrity Who is Spider-Man's new villain? Explore the top suspects like Mr. Negative, Kingpin, and Mephisto, and why a recent trailer may reveal the truth. April 4, 2026 AceShowbiz - When speculating about the main villain of Spider-Man: Brand New Day, Marvel fans have floated several suspects from Peter Parker's extensive rogue's gallery. Among the frequently mentioned contenders are Tombstone, Mephisto, Kingpin, Jackal, the Sinister Six, and Mr. Negative.